CD Chex cells
Tom Mchugh (mchugh@jekyll.ucsf.EDU)
Wed, 19 May 1993 12:52:33 -0700
We use the CD Chex cells routinely for lymphocyte antigens. The standard
antigens (2,3,4,5,7,8,19,20, etc.) seem to work fine. We have tried CD 13, 14
and 33 and they have worked. I have not noticed that the background
fluorescence is very high but we have not done this much and I will go back
and look. We have seen some unusual staining with these cells, for example
some vendors anti-CD 10 stain the cells and a target value is listed for CD
10. BD's anti-CD 10 does not stain the cells and CD says the staining for CD
10 is non-specific yet reproducible. Some other less commonly used antigens
also stain to different levels of acceptance.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
