leukocyte control ques. (re-stated)
IRBH000 (@VM.CC.PURDUE.EDU:IRBH@USOUTHAL)
WED 19 MAY 1993 10:56:00 CDT
There may have been some mis-understanding as to the point of my
question concerning the CD-Chex control cells. If so, I would
like to re-state it.
We need to know that if we analyze a clinical specimen (blood,
bone marrow, etc.) for the presence of cells of the monocytic or
myelocytic lineage, and we do not get reactivity with antibodies
(My4, My7, My8 or My9) specific for these cells, how can we be
certain the antibodies are "good" and the cells are truly non-
reactive. Instead of using a sample from a normal human control,
we had hoped that the CD-Chex control cells would provide a source
of monocytes and granulocytes that are known to react with these
monoclonal antibodies in a fashion similar to normal cells. The
manufacturer of CD-Chex cells advertises that these cells will, in
fact, react with these antibodies. However, on two occasions when
we have analyzed the granulocytes (by light scatter) in the CD-Chex
sample for reactivity with My4 (CD14), My7 (CD13), My8 (CD11b), or
My9 (CD33), none of these antibodies were reactive.
My question for the user group is, "Is anyone using these
CD-Chex control cells? If so, has anyone had a similar problem?
One other comment - the control (background) fluorescence of the
granulocytes in the CD-Chex control cells seems to be extremely high.
Has anyone else noted this? Since BD markets CD-Chex control cells,
I'm sure they must be OK and yet we can't figure out what the problem
is. Any comments would be appreciated.
Thanks,
Ray Hester
Univ. South Alabama
FAX: (205) 460-6073
Voice: (205) 473-4951
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