RE: Bird blood and lizard blood
kukuruga%kasle1.dnet@rocdec.roc.wayne.edu
Thu, 29 Apr 93 16:45:53 -0400
If you are having problems "as the samples are being run", its seems more
likely you problem lies in the instrument, unless you have reason to
believe otherwise....perhaps more details on your protocol will shed more
light.
Points to ponder... 1) Bird RBCs may be nucleated (hence the "CEN" DNA
standard); this may effect your overall analysis approach. I don't know
about lizards, but considering the link, this may be true for them also.
2) One might be well-advised to consider the existance of naturally-occuring
DNA configurational anomolies (eg. polyploidization, as in fishes)...these
may also be sources of variability. 3) DNA content staining protocols can
be extremely cell-type, and animal-source specific. Therefore, procedures
which work well on mammalian cells may prove disastrous for your samples.
MAK.
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