Free PC FACS Time Slicing Kinetics Software

ERIC MARTZ (emartz@titan.ucc.umass.edu)
Sun, 20 Sep 92 18:28:20 EDT

Version 2.1 of the free PC program MFI (Median Fluorescence Intensities) has
been posted for retrieval by anonymous FTP from flowcyt.cyto.purdue.edu
(directory pub/martz, get file MARTZEXE.ARC).

For a short introduction to version 2.0, get the file MFI200.DOC by FTP.

Version 2.1 adds:

TIME SLICING. A single data file (up to 250,000 events) can be sliced into
pieces of specified duration in seconds for kinetic studies. Histogram files
can be created for each slice for plotting purposes.

CONTROL FILE DESIGNATION. A single file per run can be designated as a
control file. Its fluorescence intensities (linearized or KMESF'd as
applicable) are corrected from the corresponding values for each fluorescence
parameter of subsequent files in the run.

GATES are still "rectangular" but are handled with less fuss. There are now
options to clear one or all gates. Gate names can now be up to 58 characters
long. Up to 8 gates can now be stored at once, from which one (or none) must
be selected per run.

There are a number of minor enhancements such as more consistent use of
ESC, warning about what ESC will do in a given context, and a menu from
which to select help topics directed to the screen.

-- 
/* - - - - - - - - - - - - - - - - - From - - - - - - - - - - - - - - - - - -
Eric Martz              emartz@titan.ucc.umass.edu    Professor of Immunology
                    Voice 413-545-2325, FAX 413-545-1578
    Morrill IVN Rm 203, Dept Microbiol, Univ Mass, Amherst MA 01003 USA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - */


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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu