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>Dear Nettrons:
>
>Has anyone tried (or heard of anyone trying) to analyze and sort yeast via
>FACS. I have heard of someone doing so on yeast protoplasts, but how
>about whole cells? Are there any particular problems with it that I need to
>address before getting started?
>
>TIA,
>Rich Buckholz
>-+-+-+-+-+-+
Some articles using flow cytometry on yeast (not protoplasts):
R. A. Preston, R. F. Murphy, and E. W. Jones (1987).
Apparent Endocytosis of FITC-Dextran by Saccharomyces cerevisiae
Reflects Uptake of Low Molecular Weight Impurities, not Dextran.
J. Cell Biol. 105:1981-1987.
R. A. Preston, R. F. Murphy and E. W. Jones (1989). Assay of Vacuolar pH
in Yeast and Identification of Acidification-defective Mutants. Proc.
Natl. Acad. Sci. U.S.A. 86:7027-7031.
C. T. Yamashiro, P. M. Kane, D. F. Wolczyk, R. A. Preston, and T. H.
Stevens (1990). Role of vacuolar acidification in protein sorting and
zymogen activation: a genetic analysis of the yeast vacuolar proton-
translocating ATPase. Mol Cell. Biol. 10:) 3737-3749.
For review including some flow cytometry, see:
Pringle, J.R., Preston, R.B., Adams, A.E.M., Stearns, T., Drubin, D.G.,
Haarer, B.K., and Jones, E.W. (1989). Fluorescence microscopy methods
for yeast. Methods in Cell Biology 31:357-435.
Robert F. Murphy
Associate Professor, Biological Sciences
Carnegie Mellon University
murphy@a.cfr.cmu.edu
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