Re: Quantification of bcl-2 by flow cytometry
TIM KUTE (tkute@bgsm.edu)
Fri, 20 Sep 1996 14:12:25 -0400 (EDT)
Dear David,
It sounds like you have put alot of time in this and I have seen
the same thing with other markers. Have you tried to add pure bcl-2 or an
extract that has bcl-2 to your antibody prior to adding it to the cells.
If you still get the same rel. fl. value than I would be leary of the flow
cytometry results. You might also try a low concentration (sub saturation
) of antibody and see what happens. Have you looked at the cells after
they are put through the flow and do they still show the same fl. intensity
in a microscope?
Let us know how it turns out.
Dr. Tim Kute email: tkute@bgsm.edu
Associate Professor in Pathology Tel#: 910-716-2950
Wake Forest Univ-Bowman Gray School of Medicine Fax#:910-716-7595
Medical Center Blvd
Winston-Salem, NC 27157-1072
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
