Sensitivity
Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
16 Sep 96 12:24:23 EDT
Hello Flowers,
Could I initiate a controversy on how people would look at very small changes
in fluorescence intensity between cells grown under different conditions? The
problem is complicated by the fact that the stained cells in question are much
brighter than the unstained controls, but stained cells (under different
growth conditions) are only slightly different from each other. How would you
set up the cytometer? Would you forget about the unstained controls? Would
you use log or lin amplification? What system would give the *greatest
sensitivity* taking all things into consideration?
Thanks.
Alice Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, NH 03756 USA
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