Multiple Myeloma - DNA labelling index

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An investigator has asked me to look into DNA labelling index analysis for
multiple myeloma patients. There is literature which suggests flourescent
labelling of plamsa cells, along with BrDU, and counting the S phase plasma
cells, is very prognostically significant.

The problem is that the investigators have been told that this can only be
done by flourescent microscopy - "it doesnt work on flow" - Naturally I
reject any such statement as ill-informed :-)

I hope I'm right ... can anyone help me with a flow based protocol ??

Thanks in advance

Peter Chapple
Senior Scientist
Peter MacCallum Cancer Institute
Melbourne AUSTARLIA

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu