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For example, 70 um, 27,500 Hz, 12 drops, 3 volt amplitude, the distance
will be around 3.8 mm.
http://facs.scripps.edu/optplus.html
See also:
Flow Cytometry: Intrumentation and Data Analysis,Eds. Van Dilla, Dean, Laerum, &
Melamed, 1985, Academic Press.
Based on the above, I see about TWICE the deflection distance with the optimal
droplet formation than what I see with BDIS settings.
This does slow down the Jet Stream, so sort speed may be slightly effected.
Dennis
*****************************************************************************
* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
*****************************************************************************
_____________________________ Reply Separator _________________________________
Subject: Distance between sort streams
Author: yagrawal@messi.uku.fi at @UCSD
Date: 9/12/96 8:25 AM
Hi everybody,
I request some advice regarding sorting.
Question: We use a FACStar for sorting. However, inspite of my best
efforts I am not able to get the Left and Right Streams to separate much
from the center stream. As a result the sorted cells miss the collection
tubes. (The left and rt. stream deflection controls are turned to max).
- Does the angle between the 2 charged metal plates (which is adjustable)
have anything to do with it ?.
- Any other suggestions would be welcome.
Many thanks in advance.
Yash Pal Agrawal
Dept. of Clinical Chemistry,
Kuopio Univ Hospital,
Finland.
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>Date: Thu, 12 Sep 1996 08:25:20 +0300 (WET)
>From: Yashpal Agrawal <yagrawal@messi.uku.fi>
>To: Cytometry M
>Subject: Distance between sort streams
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