Re: CyChrome & PerCP

Keith Bahjat (Kbahjat@nwu.edu)
Thu, 5 Sep 1996 20:03:12 -0600

To pour a bit more fuel on the fire, having used both CyChrome and PerCP, I
also would choose PerCP. Although CyChrome does give a brighter signal,
problems with Cy5 degrading (leaving only PE and a compensation nightmare)
as well as the reported binding of Cy5 to monocytes (a Cy5 receptor, if you
will) make PerCP look like a good choice. You must be selective when
choosing which Abs to conjugate with PerCP. Things like CD19 are not dense
enough to give adequate separation. As for cost, you must weigh quality vs
cost......Then sign a reagent contract and get a big discount. Life in the
90's.

Keith Bahjat
Chicago, IL

>Well, we have used both CyChrome and PcP, and we like PcP. CyChrome was
>very bright and made compensation a nightmare, whereas PcP was dimmer,
>yet this resulted in much easier compensation. BD FACscan one laser is
>the machine we use.
>CAVEAT: PcP is VERY expensive.
>cya
>BZ
>ps your milage may vary, I was told that noone in thier right mind would
>want to use the "dimmer" PcP, yet the results we get are very nice thank you.
>
>
>
>
>
>On Wed, 4 Sep 1996, steven micko wrote:
>
>> Date: Wed, 04 Sep 96 09:02:46 EST
>> From: steven micko <steven_micko@email.eushc.org>
>> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>> Subject: CyChrome & PerCP
>>
>>
>>
>> Can anyone give me information on the chemical structure (in a general
>> way), excitation/emission information, and stability information on the
>> two fluorochromes, CyChrome (Pharmingen) and PerCP (B-D)? I'm not
>> interested in violating anybody's proprietary information. I just want to
>> know what I'm dealing with. So far all I have been able to get is
>> marketing stuff (i.e. ours is better than theirs).
>>
>> Is there anything in the literature?
>>
>> Thanks in advance.
>>
>> Steve Micko
>> Emory Hospital
>> Atlanta
>>

--
Keith Bahjat
Northwestern University
Kbahjat@nwu.edu


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