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Other site specific modifications were done to enhance the TSO's daily
monitoring and setup.
For the first time in 9 months the system can be turned on, the same DDF
used, same System pressure etc and obtain the same Drop Delay as the past
few days. I can now obtain purities >99% at rates of 10k/sec plus. Sort
streams are visable from 5 feet away (tight streams).
A test sort last Friday using a ficolled Bone Marrow sample of about 15
million cells was stained with CD3-Fitc, CD34-PE, CD45-PCP. CD3+CD45+
and CD34+CD45+ cells were sorted into seperate tubes. The sample was
sorted at a rate >10k/sec. The initial CD34 % was 1.3 and the CD3 was 7.7.
The sorted CD3 were >98% pure. The CD34 sorted cells were >95%. The
recovery was about 52%. These results were better than expected with the
problems I had been experiencing with this system most of this year.
The problem is fixed. I just need to tweak it.
I would like to hear from anyone else with a TSO or who is going to
purchase one.
Thanks to BD , Alfonso discovered the heat problem and John Cardot and
Larry Duckett modified the system to meet my particular needs.
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