Re: Protocols for fluorescent conjugations (Ig or other)
Jim Zanghi (zanghi@merle.acns.nwu.edu)
Wed, 14 Aug 1996 15:04:34 -0600
I recently picked up a book entitled, "Antibodies: A Laboratory Manual" by
Harlow and Lane (Cold Springs Harbor, 1988) available from Sigma for $70.
It covers the basics about antibodies, addressing some of the questions I
recently asked here, and one chapter discusses antibody conjugations,
including enzymes, biotin, and fluorochromes. Avidin conjugations are not
included, and this book does not discuss flow cytometry, but it is very
straight forward and ideal for beginners.
Jim
...truth is marching in
- Albert Ayler, a free jazz musician
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu