Ca flux in adherent cells

colette charland (ccharlan@zoo.uvm.edu)
Wed, 24 Jul 1996 13:21:38 -0500

Need I say more? A visiting investigator wishes to modulate Ca flux
in an adherent cell population. As a pilot, he had tried loading the cells
with Indo while they were still attached, trypsinizing the cells and then
"hitting" them with ionomycin. This was unsuccessful. For a second
attempt, he tried a reagent (that was suggested over this bulletin board-
for use with endothelial cells) which he said gently removed the cells, then
loaded with indo and then tried stimulating with an agent that he has
demonstrated is in fact effective for these cells. He was still unsuccessful.
The question is, can Ca flux be done on adherent cells and then
passed through the flow?

Thanks in advance for all replies

Colette Charland
University of Vermont

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu