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an investigator here has been studying the effect of various peptides on er
negative cells (monocytes and dendritic cells).
our question is whether using ionomycin (as our positive control/at various
concentrations) should the ratio of violet to blue return to near baseline
after the influx of calcium?
we are loading with indo-1 and are using an argon ion laser tuned to UV.
we get increases in ratio of violet to blue of about 4:1 after stimulation but
this stays high even for 10 minutes.
this is my first time doing calcium flux experiments and i would be very
grateful to hear from anyone with some comments.
thank you very much.
frank isdell
flow cytometry
the rockefeller university
1230 york ave
ny 10021
tel 212-327-7657
fax 212-327-8875
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