RE: nozzle vs particle size
David Galbraith (dgalbrai@ag.Arizona.EDU)
Wed, 10 Jul 1996 09:33:01 -0700
Be aware that the larger the nozzle, the slower the max sort rate (and the
larger the sheath tank!). We have shown that it is possible to sort 95 um
pollen with a 155 um tip, with sort efficiencies >80%, if you employ a long
enough undulation wavelength and take care in alignment. A full description
can be found in Cytometry 8:60-70 (1987).
David Galbraith
At 04:15 PM 7/9/96 -0400, you wrote:
>
>Simon Monard noted:
>> 50u mesh sounds a bit big for a 70u nozzle.
>
>Indeed, BDIS recommends using nozzles with a diameter that is four times
>larger than the diameter of the largest particle sorted! A 200 micron tip
>is available for the MacroSORT option (largest nozzle is 400 microns). For
>a 70 micron tip, I use a 20 micron mesh; admittedly not quite 4:1, but at
>least it gets out the visible clumps, pieces of tissue, etc. :)
>
>/\/\/\_ Eric Van Buren, vanburen%flovax.dnet@rocdec.roc.wayne.edu
>\ \ \ Karmanos Cancer Institute and Wayne State University,
> \_^_/ Immunology & Microbiology. Detroit, Michigan.
>
>
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