FW: BAL AND CLOGGING

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I OPERATE A FACSTAR+ AND ON OCCASIONS I RUN SUPER TRASH SPECIMANS. OF
COURSE
WE FILTER [SOMETIMES 2X], AND WE
WILL USE ANTI-COAGULANT OF CHOICE. WHAT I FOUND HELPS ME THE MOST IS AS FOLLOWS:
1. LOWER YOUR THRESHOLD TO 20. THIS GIVES UOU A REALISTIC EVENT COUNT
OF WHAT
IS PASSING THROUGH YOUR 70
MICRON APERATURE. [COULD YOU USE 100u ?]
2. LOWER YOUR EVENTS /SEC. TO APOX. 2K.
3. BACK FLUSH [SAMPLE CHANGE] EVERY 30-45 MIN..
4. IF THINGS GET REALLY BAD DON'T BE SQUIMISH ABOUT USING 20% CLOROX FOR
10 MIN
FOLLOW WITH A D.WATER RINSE.

I REALIZE THIS DOSE NOTHING FOR THE THE DEUL PLOT VISUAL PROBLEM. HERE
YOU MAY
WANT TO MAKE A SINGLE FSC
HISTOGRAM UNGATED.

GOOD LUCK

HOWARD MOSTOWSKI
FDA/CBER [NIH CAMPUS]
MOSTOWSKI @A1.CBER.FDA.GOV
301-827-0704

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu