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NaCl (<1%)
di-water
2-phenoxyethanol
sodium phosphate dibasic
potassium phosphate monobasic
It is my understanding that the 2-phenoxyethanol has bacteriocidal
properties (Merck Index). I have seen very few instances where the
preservative has somehow gotten in the way of performing flow cytometric
analysis. ONE EXCEPTION HOWEVER WAS IN SORTING...ON THE BD
FACSORT...when comparing the results of a sort using sterile 1x PBS vs.
the commercially available sheath fluid (a la preservative), I noticed
that there was a more significant portion of dead cells when sorting with
the phenoxyethanol laced fluid.
Hope this helps....
SIncerely,
Seong-Joo Jeong, Technician
University of Pennsylvania Cancer Center
Flow Cytometry and Cell Sorter Shared Resource
297 John Morgan Building
3620 Hamilton Walk
Philadelphia, PA 19104-6082
email: sjeong@pobox.upenn.edu
Phone: 215-898-3528
FAX: 215-898-4227
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