Endothelial cells by flow
DODONNEL@svherc.ucd.ie
Wed, 03 Jul 1996 15:06:39 +0100 (BST)
Has anybody out there any experience looking at endothelial cells ? A
researcher in our lab asked me to look at some of hers (HUVEC line) with a
view to examining expression of adhesion markers in the future.
Although on microscopy they seemed to be a population of fairly large cells
of quite uniform size, on the FSC/SSC plot there seemed to be 2 populations,
one with moderate to high FSC and SSC increasing proportionately,
the other with a lower SSC but an FSC going off the scale.
I wondered if the 2nd lot represented aggregated cells, although she did
trypsinise the cells before fixing them. Both populations had the same
autofluorescence characteristics.
I'd be very grateful for any words of wisdom.
Dr. Dearbhaile O' Donnell
University College Dublin
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
