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An alternative to AMCA as a UV-excitable is Cascade Blue, obtainable
from Molecular Probes in the form Neutralite Avidin Cascade Blue, and
claimed by them to be brighter than AMCA. In our hands, the
signal-to-background was decades lower than for an equivalent FITC
conjugate, but it may be good enough for a dense surface receptor.
Cascade blue would really like to be excited near 400nm rather than
the Argon 350-360nm we used.
\ / < Flow Systems Laboratory
Frank Battye \__/ <<<<< The Walter & Eliza Hall Institute
Robyn Muir ----------!!<<<<<<<< Voice: 61_3_9345 2540
Dora Kaminaris /!!\ <<<<< Fax: 61_3_9347 0852
o !! \ < IN:: "facs_copy@wehi.edu.au"
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