Mouse flow

Jeff Carrell (Jeff_Carrell@hgsi.com)
Mon, 01 Jul 96 14:06:22 EST

If anyone routinely does flow on peripheral blood from mice and could offer
advice, hints or tricks, I could really use the help.

We are using a BD FACScan. We are currently using Gentrak lyse/fix, although I
think we're going to try the BD stuff.

I'm looking simply at what changes occur in the basic lineages (mono, gran,
lymph) when the mice are treated with novel proteins. I am using a three tube
panel: isotype control, B220/CD3 and CD14/Gr.1 (two color).

I'm more familiar with human LWB; the mouse FSC v. SSC looks similar but the
monocytes aren't as clearly separated--is this usually the case with mice?

Any mouse flow hints would be great,

Thanks,
Jeff Carrell
e-mail:
jeff_carrell@hgsi.com


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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu