Re: quantitation of fetal cells by flow
Bruce H. Davis, M.D. (bdavis@beaumont.edu)
Mon, 01 Jul 1996 13:50:42 -0700
Regarding the discussion of late on fetal cell detection, I can add that
we have recently developed a method (yes Howard, the patent is now
filed) using 3 monoclonal antibody clones to hemoglobin F (fetal) and a
permeabilization method using FCM. We get ~2 log separation between
maternal cells and fetal cells and have further developed an
data analysis routine using Verity's Modfit to reproduceably quantitate
fetal cells, so-called F-cells (RBCs with HbF and HbA seen in "stressed"
erythropoiesis), and adult cells. This method will be presented at the
Charleston Clinical Cytometry meetings in August and the International
Society for Hematology meeting in Singapore in August. The clones and
method are available for licensure and for collaborative studies. For
more information come to Charleston, Singapore or call, fax or write.
Bruce H. Davis, M.D.
****************************
Director of Analytical Cytometry
Dept. of Clinical Pathology
William Beaumont Hospital
3601 W. 13 Mile Rd.
Royal Oak, Michigan 48073-6769
810-551-5137
FAX 810-551-8057
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
