Re: quantitation of fetal cells by flow

Andreas Radbruch (andreas@gen1.genetik.uni-koeln.de)
Wed, 3 Jul 96 17:48:19 +0100

We and others have used MACS as a quick, cheap easy to handle and very
efficient method to enrich fetal cells from maternal blood, using the same
parameters and obtaining the same purities and recoveries as others have
with FACS.
The reference is Buesch, Huber, Pflueger, Miltenyi, Holtz and Radbruch
(1994), Enrichment of fetal cells from maternal blood by high-gradient
magnetic cell sorting (Double MACS) for PCR-based genetic analysis; Prenatal
Diagnosis 14, 1129-1140.
best regards, Andreas Radbruch

>
>The query to which you replied was the 2nd or 3rd casual request I've seen
>on the Mailing List for a pat answer to an unsolved and very difficult
>problem. Fetal cells in maternal blood are very rare events, and there are
>several top rate labs - Diana Bianchi's in Boston and Dorothy Lewis' in
>Houston come to mind - working really hard to develop methods which will
>probably be practical only for almost equivalently high powered labs. Yes,
>fetal cells are different in their membrane and antigenic characteristics,
>and they have been isolated (the first confirmatory PCR, identifying
>Y-chromosome-bearing fetal cells, was done in Diana's all-female lab,
>eliminating the possibility that a flake of male dandruff would produce a
>false positive result). There is a recent volume of Annals of the New York
>Academy of Sciences largely devoted to the topic. I don't think there's a
>brand new, quick and dirty method out there; anything which was discovered
>in the past year is likely to remain secret until patents are filed.
>-Howard
>
Andreas Radbruch


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu