Re: fixing and storing cells
Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
13 Jun 96 11:13:47 EDT
Hello Ruedi,
My general impression (but you should probably check it yourself) is that PE
and/or FITC-stained cells fixed in 1% paraformaldehyde (if kept cold and dark
after fixation) are very stable and can be read reproducibly after several
weeks or more. However, there are changes that occur rapidly upon fixation
(background fluorescence goes up a bit, PE fluorescence goes down a bit, some
cell types (eg granulocytes) change their scatter characteristics
significantly). Therefore, we always keep cells for at least 24 hours after
fixation before we analyze them -- to give time for these changes to stabilize.
Hope this helps.
Alice
Alice L. Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, New Hampshire
NH 03756 USA
tel 603-650-7661
fax 603-650-6130
e-mail givan@dartmouth.edu
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