Platelets activation -Reply
Barry Udis (UDIS@brody.med.ecu.edu)
Fri, 31 May 1996 10:54:06 -0400
I asked our resident Platelet expert and his reply is as follows:
"Add Prostaglandin E-1 to the anticoagulant in the sample(not in
the patient, please) at 300 nM or higher concentration. This
should prevent and reverse platelet clumping."
Barry Udis
>>> Osama Nabulsi <Osama.Nabulsi@amgen.com> 05/30/96
06:34pm >>>
REGARDING Platelets activation
Dear Fellow Cytometrists:
We are running a leukapheresis (LP) blood assays on the flow
cytometer and in some cases we encounter platelets activation
problems where we are unable to gate them out especially if
they cross link WBCs.
What are the different procedures to stop platelets from being
activated during handling?
Osama Nabulsi
Amgen Inc.
(805)447-1594
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
