re:re: intracellular IL4 and IFNg

Mikael Vestberg (Mikael.Vestberg@inflam.lu.se)
Fri, 31 May 96 18:00:50 +0200

We have done analysis in mouse lymph node T cells=20
after in vitro culture. After 4 days or more of=20
culture w ConA and then a 6 hour PMA/ionomycin=20
treatment in the presence of monensin we see=20
abundant IFN gamma and some IL4. They are not=20
expressed in the same cells.

We use 50 ng/ml ConA and 50 ng/ml PMA and 1 ug/ml=20
ionomycin in the presence of 3uM Monensin.

Mikael

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu