Re: 4 color flow
KH Leong (nmilkh@leonis.nus.sg)
Tue, 28 Nov 1995 08:38:17 +0800
Mike,
We also use an Elite ESP for 4-colour analysis, however, we use ECD (ie.
FITC, PE, ECD & PerCP) instead of APC so that all the fluorochromes are
excitable with one laser (488 nm). Fluorescence is collected on PMT2-5, and
compensations range between 0.2 to 46.7% (details upon request)...works
everytime! We are now looking at dyes emitting in the infrared and far uv
regions for the 5th and 6th colour analysis. Hope this help.
>We are using an Elite ESP with a gated Amp. Can FITC, PE, PerCP and APC work
>together successfully? PerCP is on the 5thPMT upper beam and APC on the
>lower beam. What about compensation?
>
>Thank You
>Mike
>American Red Cross
>BMT Department
>
>
K.H. LEONG
National University Medical Institutes
National University of Singapore
BLK MD11 #02-01 Clinical Research Centre
SINGAPORE 119260
Phone: 65-7728066 Fax: 65-7735485
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