Re[2]: HIV viral load and flow cytometry: methods & perspect

Dennis_Young@CIS.ucsd.edu
Mon, 13 Nov 1995 15:46:00 -0800

The reference:
Patterson, B.K., Till, M., Otto, P., Goolsby, C., Furtado M.R., McBride, L.J.,
Wolinsky, S.M., Detection of HIV-1 DNA and Messenger RNA in Individual Cells by
PCR-Driven in Situ Hybridization and Flow Cytometry, Science, Vol. 260, 14 May,
1993, pp. 976-979

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______________________________ Reply Separator _________________________________
Subject: Re: HIV viral load and flow cytometry: methods & perspective
Author: rh208@cus.cam.ac.uk at @UCSD
Date: 11/13/95 11:03 AM

Hi Bjarne,
There was a paper on PCR-Driven flow cytometry in Science (november
1993 I think), where the researchers claim that they could detect singly
infected cells. I'm afraid that I'm in the process of moving house (I hope),
and my copy of the paper is in a pile of boxes, so I can't give you too
much detailed information, I hope that this is enough to go on.

Ray

On Sat, 11
Nov 1995, Bjarne Moeller wrote:

> The clinicians at our center has confronted me with a few relatively
> simple questions, which I am not able to answer and therefore has
> forwarded to the List of Flow'ers:
>
> We have been wondering if anybody has used flow cytometry to count the
> number of infected cells in HIV-1 patients. Any communications of
> correlation to molecular biology (ie. semiquantitative HIV-1 RNA-PCR)?
>
> If so, is the infected fraction predictive of clinical outcome of HIV-1
> infected patients?
>
> _________________________________________________________________
>
> Bjarne K. Moeller, MD
> Dept. of Clin. Immunology Voice: +45 8949 5304
> University Hospital of Aarhus Fax: +45 8949 6007
> Skejby Sygehus
> DK-8200 Aarhus, Denmark E-mail: bkm@biobase.dk
>
> _________________________________________________________________
>
>
>

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