RE: Background Subtraction

Doug Smoot (rin0dss@bumed30.med.navy.mil)
Thu, 9 Nov 1995 11:05:53 -0400 (EDT)

Roy Overton, in the November 1988 edition of Cytometry published a
subtraction algorithm to do background subtraction. This subtraction method
was supposed to deal with the question of shifting "negative" peaks and give
a more "accurate" positive/negative result. In the July 1989 issue (p.492)
there is a correction to the PASCAL source code he provided in the software.
The Coulter Elite software incorporates this subtraction protocol in its
Immuno-4 analysis page. I believe Phoenix Flow's MultiPlus includes a
version of this subtraction algorithm as well. I hope this helps.

Doug Smoot
{---------------------------------------------------------------------}
Douglas Smoot
Naval Medical Research Institute, Code O613
Bethesda, MD 20889-5607
voice : (301) 295-1121 fax: (301) 295-6857

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