fixative/HIV+samples/Autolyse

Hughes, EJ, Jane, Ms (JHUGHES@ich.uct.ac.za)
Thu, 9 Nov 1995 14:12:00 SAST-2

Hello
I have been comparing different lysing agents and was wondering if it
was possible and/or advisable to add paraformadehyde to directly
stained whole blood from HIV positive patients subsequently lysed
using B-D Autolyse if one used this reagent on the bench instead of
at a FacsPrep workstation.

Could anyone please comment as the price per test would be very
competitive to Immunoprep . Another thing, is Paraformaldehyde the
fixative of choice, is Formalin not a good idea and if so why?

Thanks
Jane

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