fixative / HIV+ samples / B-D Autolyse

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• Next message: Dennis Broud 301-594-6259 FAX 301-443-9292: "Beyond gating revisited"
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I have been comparing different lysing agents and was wondering if
it was possible and/or advisable to add Paraformaldehyde to directly
stained whole blood from HIV+ patients subsequently lysed using B-D
Autolyse if one used this reagent on the bench instead of in the Facs
prep workstation. The final volume is 2ml and I was considering
adding 200ul of a 10% Paraformaldehyde.

Could anybody please comment as the price per test would be very
competitive to Immunoprep and I think I'm right in that these are
the only 'no-spin' lysing reagents available. Another thing, is
Paraformaldehyde the best fixative for HIV positive samples, is it
better than Formalin and if so, why?

Thanks

Jane

• Next message: Dennis Broud 301-594-6259 FAX 301-443-9292: "Beyond gating revisited"
• Previous message: Eric Martz: "RE: general purpose siftware for gating"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu