Re: your mail
Bruce Davis, MD - Clinical Pathology (bdavis@beaumont.edu)
Wed, 25 Oct 1995 10:24:41 -0400 (EDT)
I have developed a flow method using some monoclonal antibodies developed
in my laboratory with a >two log separation of fetal or neonatal cells
from adult RBCs, resolution to 0.1% and simplied data analysis using
Verity Software's Modfit to model % neonatal hgbF containing cells and
so-called F-cells with lower levels of Hgb F. My goal is to
commerciallize the monoclonals and method and currently in the earlier
stages of this, so I can not give you a estimate of when such an assay
will be commercially available. There are also other published methods in
the literature, such as S. Thorpe et al, Brit J Haematol 87:125-132, 1994,
Horiuch et al, Cytometry 20, 261-76, 1995 (image method), and Medearis et
al Am J Obstet Gynecol 148, 290-295, 1984. Hope this info helps.
Bruce H. Davis, M.D.
Wm Beaumont Hosp.
Royal Oak, MI 48073
810-551-5137
FAX 810-551-8057
On Tue, 24 Oct 1995, ILAB Husebekk Anne wrote:
>
> I would like to ask if anyone make detection of fetal cells in maternal
> bloodsamples using flowcytometry?
> Anne Husebekk, ilabah@rito.nor
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
