CD34 measurement

Ian Titley Leukaemia Reaserch Fund Centre (ian_t@icr.ac.uk)
Wed, 25 Oct 95 15:27:47 +0100

Hi

Further to Ed's request about how people measure CD34+ cell numbers by flow.
As well as asking about whole blood vs mononuclear preps and whether to use
CD45 or not (and indeed which CD45 antibody) it would also be interesting to
know which clone of CD34 antibody people use (since there are at least three
distinct epitopes on the CD34 molecule).

Thanks

Ian Titley
Leukaemia Research Fund Centre
at the Institute of Cancer Research
237 Fulham Road
London

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