Re: FACScan cleanup - Washing solutions ???

/G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Tue, 24 Oct 1995 05:14:00 -0400

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We use a 10% Domestos solution made up with distilled water
and sterilefiltered. This we keep for up to 3 month in the
dark at room temperature without problems. Domestos contains
15% hypochlorit (in the UK market). It forms a precipitate
with (hard) tab water wich you would not want in the FCM.

Gerhard Nebe-v.Caron
Unilever Research, Colworth Laboratory Sharnbrook,
Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
E.mail: gerhard.nebe-von-caron@urcgb.sprint.com

______________________________ Reply Separator _________________________________
Subject: FACScan cleanup - Washing solutions ???
Author: mhaury@pasteur.fr at INTERNET
Date: 23/10/95 22:34

Hello..

I'm just interested in the current washing - cleanup solution that people
use for cleaning their machines. Our FACScans systematically get clogged up
because the hypochlorite solution (supposedly 1 %) forms cristals in the
stock solution.. the only solution is to prepare fresh every day (which I'm
too lazy to do...).

Is there anyother detergent/solution that people use to cleanup that
doesn't have this problem ??

Thanks a lot,

Matthias
______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (1) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (1) 45 68 86 39 INSTITUT PASTEUR PARIS
______________________________________________________________

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu