Re: FACScan cleanup - Washing solutions ???

Clare_Louise Hughes (hughes@icrf.icnet.uk)
Tue, 24 Oct 1995 16:53:12 +0000 (GMT)

Hello there,in general we clean the fluidics once a week with a 1:250
solution of 7x for approx 30mins,being careful to by pass the sheath
filter of course and following that distilled water,and then back to the
pbs.As we run a fair amount of clumpy dna cells, we also put through a
solution of bleach once a month(1:10).I hope that this has been of some help
to you,clare..

Clare Hughes-ICRF.

On Mon, 23 Oct 1995, Matthias Haury wrote:

> Hello..
>
> I'm just interested in the current washing - cleanup solution that people
> use for cleaning their machines. Our FACScans systematically get clogged up
> because the hypochlorite solution (supposedly 1 %) forms cristals in the
> stock solution.. the only solution is to prepare fresh every day (which I'm
> too lazy to do...).
>
> Is there anyother detergent/solution that people use to cleanup that
> doesn't have this problem ??
>
> Thanks a lot,
>
> Matthias
> ______________________________________________________________
> Matthias Haury _/_/_/ _/_/_/ _/_/_/
> Flowcytometry - Immunology _/ _/ _/ _/ _/
> Institut Pasteur Paris _/ _/_/_/ _/_/_/
> Email: mhaury@pasteur.fr _/ _/ _/
> Tel: 33 (1) 40 61 31 29 _/_/_/ _/ _/
> Fax: 33 (1) 45 68 86 39 INSTITUT PASTEUR PARIS
> ______________________________________________________________
>
>
>

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