detection of lacZ (fwd)

Zev Silverman (szeev@bgumail.bgu.ac.il)
Tue, 24 Oct 1995 14:57:02 +0200 (IST)

---------- Forwarded message ----------
Date: Mon, 23 Oct 1995 22:50:19 +0200 (IST)
From: Zev Silverman <szeev@bgumail.bgu.ac.il>
To: cyto-inbox
Cc: szeev@bgumail.bgu.ac.il
Subject: detection of lacZ

I wonder whether there is a way to detect reporter genes in cells from
transgenic animals via FACS? I have recently seen a catalogue for an in
vivo lacZ B-galactosidase detection kit and would like to be able to
separate lacZ-containing from lacZ-negative cells for primary cultures.

Is there a reporter gene which codes for a membrane protein that can be
labeled without fixing a cell?

Thanks in advance for any help you can offer,

Ze'ev Silverman

_________________________________________________________________________
Ze'ev Silverman, Ph.D.
Zlotowski Center for Neuroscience
Faculty of Health Sciences, Ben-Gurion University of the Negev
Beersheva 84105 Israel

Phone: 972-7-400891 FAX: 972-7-277655 email: szeev@bgumail.bgu.ac.il
_________________________________________________________________________

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