Re: Fellow CellQuest users

Mikael Vestberg (Mikael.Vestberg@inflam.lu.se)
Thu, 19 Oct 95 21:13:53 +0100

>Date: Wed, 18 Oct 95 10:24:13 PDT
>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>From: Mailer-Daemon@aphid (Mail Delivery Subsystem) (by way of geri=20
>pingul <Mailer-Daemon@aphid>)
>Subject: Fellow CellQuest users
>
> =20
>We have been using CellQuest for approx. 1 month now and have been
>experiencing an annoying and sudden crashing of the software while=20
>acquiring
>data. The error message reads, "Application unknown has suddenly quit,
>system error #1". =20
>
>As BD has informed me that error #1 refers to a memory problem, I have
>increased the memory for the application to 6000K and am still=20
>experiencing
>the crashes. I first thought it was a problem with the acq and storage
>options applied by some of our users with rather messy samples, (ie=20
stop >acq
>after 10000 events within G1 and store all events), thereby filling RAM=20
>with
>alot of other 'junk'. But this is inconsistent with the observation=20
that >I
>can get the software to crash with this same error message while=20
>collecting
>only 4000 total events.
>
>Is anyone else experiencing this same problem and if so, have you=20
>figured
>out a solution? Is there an equivalent LYSIS direct disk storage=20
method >for
>the MAC? Will increasing the allowable RAM yet again, say to 12000K be
>beneficial? As BD is looking to fix this bug with the next upgrade, I=20
>am
>looking for a solution in the mean time. Any suggestions?
>
>Thanks,
>
>=20
>Geri Pingul
>Flow Cytometry
>Fred Hutchinson CRC
>
hello Geri,

The problem has been discussed before on the mailing list. It appears=20
that system 7.5 is not compatible with acqusition but only analysis of=20
already acquired data. I suggest you swith to system 7.1. If you=20
already are using 7.1 try to get rid of as many other inits as possible=20
from that particular mac and also disable network. Hope that helps.

Mikael

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