Agarose microdrops

Mrs L M Dash (lmd2335@ggr.co.uk)
18 Oct 95 16:20:00 BST

I would like to select transfected cells on the basis of their secreted
proteins. I have a paper (Kenney et al.) which describes the use of agarose
microdrops and cell sorting to separate hybridomas producing appropriate
antibodies.
I would be grateful if anyone out there with experience with this technique
has answers to the following questions:-

1) Do I need specialised eqipment to make reasonable microdrops? How much
does it cost?
2) How straight forward is the method of preparation and sorting with
microdrops? Are there any technical problems I can expect and do you have
any tips for trouble shooting?

Thanks in advance for your help,
Laura Dash (Glaxo Wellcome).

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu