Right angle scatter with UV laser line?

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Do you not get right angle light scatter (or very little of it) when
using the UV line? I was just setting up for an experiment in which we
will be using UV on our FACS 440. For alignment, I was using some old
Polyscience beads
(about 5 micron dia.) that still fluoresce and I didn't see much side
scatter.
Should I? Will it be different with cells, i.e., will I see SS with cells
even though I don't see it with these beads? If not, is it a property of
the laser line or the PMT?

Thanks for any answers.

Ray

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Raymond B. Hester, Ph.D. Someday, after we have mastered the
Flow Cytometry Lab winds, the waves, the tides and
The University of South Alabama gravity, we shall harness the energies
Mobile, AL 36688-0002 of love. Then, for the second time
Email: rhester@jaguar1.usouthal.edu in the history of the world,
Voice: (334) 460-6029 man will have discovered fire.
FAX: (334) 460-6073 -Teilhard de Chardin
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu