Megakaryocyte Sorting

lori_m@SMTPGATE.BCSEW.EDU
Thu, 28 Sep 95 11:29:36 CST

One of our research labs asked whether we could sort dog bone marrow
for megakaryocytes on our FACStar PLUS. Has anyone done this? If so,
what nozzle tip size did you use? What kinds of frequencies were you
using with that nozzle? Any specific prep requirements?

Are there better options than flow? We are trying to cut down on the
manual labor involved in finding/collecting the cells while
maintaining the best environment for them.

Thanks for your help.

Lorie

_________________________________________________________
Lorie Miller
Flow Cytometry Core Lab
Blood Center of Southeastern Wisconsin
lori_m@smtpgate.bcsew.edu

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu