 |
 |
 |
 |
1. Brefeldin A is MUCH better than monensin. It is less toxic and
exerts its effects in a shorter time frame--we add it to cultures typically
4 hours before staining.
2. Pharmingen is marketing a line of anti-cytokine antibodies that they
claim are optimized for intracellular staining. We have used their
FITC-anti-mouse interferon gamma and PE-anti-mouse IL4 with success.
Several other companies have labelled anti-cytokine antibodies, but they
may not be suitable for intracellular use. You'll just have to try them.
Good luck,
Jonni
Jonni S. Moore, Ph.D.
Director, Cancer Center Flow Cytometry and Cell Sorting Facility
University of Pennsylvania School of Medicine
297 John Morgan Bldg
Philadelphia, PA 19104-6082
Phone: 215-898-6853
FAX: 215-898-4227
e-mail: moorej@a1.mscf.upenn.edu
|
|
|
|