Re[2]: FCS transfer

Dennis_Young@CIS.ucsd.edu
Thu, 7 Sep 1995 14:52:00 -0700

The Fetch program defaults to ASCII (Text) file transfer mode. The FCS datafile
was converted in the transfer process to a Microsoft Word Document. (The cute
little icon shows the W for MS Word.) The original datafiles were deleted, but
the file size is the same. I can read the filesize in the FCS header! The data
is corrupted; it's not really in ASCII or FCS format. When I read the file in
WinMDI, I see semi-random events.
Joe Trotter suggests stripping carriage returns. How else does an Apple know if
a file is a Word document?

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______________________________ Reply Separator _________________________________
Subject: RE: FCS transfer
Author: emartz@microbio.umass.edu at @UCSD
Date: 9/6/95 8:22 PM

In message Wed, 06 Sep 1995 12:19:00 -0700, Dennis_Young@cis.ucsd.edu writes:

> We mistakenly transferred a FCS 1.0 file using Fetch 2.1 on the Apple and
> FACSNet on the Host HP in the Automatic mode. This mode saves files as
> text files. Can the FCS data be rescued?

If by 'text' you mean list mode data as ASCII columns of numbers
rather like a spreadsheet (or if you can convert the file to this
form), you could use my A2FCS (ASCII to FCS) program, which is
an improved version of Joe Trotter's TEXT2FCS. A2FCS.EXE is a
DOS program which is part of a test suite not yet fully integrated
into my program MFI (and hence not yet officially released):

ftp://flowcyt.bio.umass.edu/pub/flowcyt/mfi/beta.dir/mfiverif.exe
is the self-unpacking zipped file.

The test suite also includes programs for generating synthetic
flow cytometry 'data' of various sorts.

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>Subject: RE: FCS transfer
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu