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David Hurley
SDSU
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REPLY FROM: Hurley, David Biol
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To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
From: Geoff.Osborne@anu.edu.au (Geoffrey Osborne)
Subject: Ion fluxes in bacteria?
Hello,
This may sound like an unusual question but I had a request
yesterday from a user interested in detecting ion fluxes in bacteria. I
don't know of any references regarding this and wondered if someone might be
able to point me to some. I think the basic idea is to insert a sequence of
interest tagged with LacZ and detect it in those cells expressing it by
flow, and also assess the movement of things like calcium or potassium, and
hopefully see some change related to the inserted sequence.
Any and all suggestion welcome.
Thanks in advance,
Geoff
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Geoffrey Osborne | ____ __ o Ahh!
Flow Cytometry (FACS LAB) | __ `\ <,_
John Curtin School of Medical Research, | __ (*)/ (*)
Australian National University, | ==============|
CANBERRA, AUSTRALIA. | |--|
Email: Geoff.Osborne@anu.edu.au | |--|...
-----Surfing the Web?: Try http://jcsmr.anu.edu.au/facshome.html------
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