 |
 |
 |
 |
Texas Red is now available as a succinimidyl ester from Molecular Probes
(rather than the sulfonyl chloride that can be problematic when labelling
mAbs).
Both of these offer chemistries that are pretty simple (especially compared
to PE comjugation).
------------------------ Forwarded Message -----------------------
Date: 9-7-1995 7:00pm
From: {mhaury@pasteur.fr (Matthias Haury)}:unix:niaid
To: calman prussin:10:niaid,
barbara butcher:4:niaid
Subj: Protocols for PE Conjugation...
Also-to: cytometry mailing list <cytometry@flowcyt.cyto.purdue.edu>
--------------------------------------------------------------------
Hello everybody out there,
Having problems to conjugate a hamster mAb to Phycoerythrine, I'm curious
to know if there are different protocols out there for custom direct
conjugation of antibodies to PE. Are there species specific problems
(hamster vs mouse or rat mAbs) ?? I'd be happy about any protocols or hints
to improve our success... (PE is a bit too expensive for trial and
error...).
I'm also interested in other custom conjugation of fluorescent markers
(APC, PerCP, Cy5, etc...), as well as eventual suppliers of the necessary
reagents.
Thanks very much already in advance...
Matthias
______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry _/ _/ _/ _/ _/
Department of Immunology _/ _/_/_/ _/_/_/
Institut Pasteur _/ _/ _/
25 rue du Dr. Roux _/_/_/ _/ _/
F - 75724 PARIS Cedex 15 INSTITUT PASTEUR PARIS
France
Tel: 33 1 40 61 31 29 Office
Tel: 33 1 40 61 33 52 Lab 1
Tel: 33 1 45 68 85 38 Lab 2
Fax: 33 1 45 68 86 39
Email: mhaury@pasteur.fr
|
|
|
|