Re: flow crossmatch

ric thorpe (ric_thorpe@ccmail.llu.edu)
Thu, 07 Sep 95 11:59:32 PST


Joanne, in reply to your 8/18/95 email"

1. We are doing PRA's by ELISA and the results compare to flow. In the past we
have occasionally done flow PRA's with a pool of 10+ fresh cells.

2. a. LR & cadaver = yes
b. CDC = yes
c. go with "FLOW"
d. Ficolled cells or flushed lymph node cells, both T & B cells.

Linda Buckert
Loma Linda Immunology Center
Loma Linda, CA 92354
email: Linda_Buckert@ccmail.llu.edu

______________________________ Reply Separator _________________________________
Subject: flow crossmatch
Author: Joanne.Luider@CRHA-Health.Ab.Ca at InternetMail
Date: 8/18/95 2:52 PM

We need advice/information on what's happening out in the world as far as
using flow cytometry crossmatch methods vs the cytotoxicity methods (CDC).

Some of our questions are:

1. Do you screen and/or identify PRA by flow?
-do you still use/report CDC results as well?
-what do you do when the flow result is positive and CDC is negative?
_do you use pooled random donor cells to screen? Fresh/frozen?
_advantages/disadvantages?

2. Do you do flow crossmatching prospectively for renal(or other organs)
tranplants for living related or cadaver donors?
_do you still use/report CDC results as well?
-what results are used if the results differ?
-what methodology is used for flow crossmatching? ficolled cells/whole
blood lysis? T and B?

Thanks in advance everyone!

Joanne Luider
Foothills Hospital
Calgary, Alberta
phone (403)670-4765
fax (403)270-4135


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