flow crossmatch

LUIDER@Gonzo.FhHosp.Ab.Ca
Thu, 17 Aug 1995 11:46:55 -0600 (MDT)

We need advice/information on what's happening out in the world as far as
using flow cytometry crossmatch methods vs the cytotoxicity methods (CDC).

Some of our questions are:

1. Do you screen and/or identify PRA by flow?
-do you still use/report CDC results as well?
-what do you do when the flow result is positive and CDC is negative?
_do you use pooled random donor cells to screen? Fresh/frozen?
_advantages/disadvantages?

2. Do you do flow crossmatching prospectively for renal(or other organs)
tranplants for living related or cadaver donors?
_do you still use/report CDC results as well?
-what results are used if the results differ?
-what methodology is used for flow crossmatching? ficolled cells/whole
blood lysis? T and B?

Thanks in advance everyone!

Joanne Luider
Foothills Hospital
Calgary, Alberta
phone (403)670-4765
fax (403)270-4135

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu