Chemi-luminescense with flow.

Archibald S Conner (conneas@Eng.Auburn.EDU)
Wed, 2 Aug 1995 05:57:07 -0500 (CDT)

thoughts on using chemi-luminescense in conjunction with flow. We have a
method of keeping the bioluminescent product associated with the cell tagged
with the enzyme i.e. horseradish peroxidase. Can anybody suggest any
substrates that would be particularly well suited to flow i.e. have a
long lifetime of the luminescense and a bright signal.
I'm thinking of looking at these particles with the laser off and
triggering on FL1 (the light emitted by the luminescense).
Can anyone think of reasons why this won't work?

-Thanks Stewart Conner


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