FDG staining for b-gal

Rochelle A. Diamond (diamond@cco.caltech.edu)
Tue, 1 Aug 1995 18:07:14 -0700 (PDT)

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Cyto Listers-
I am about to embark on trying to stain transgenic mouse lymphocytes
carrying a b-galactocidase reporter gene with FDG for sorting. I could use
advice, protocols, and/or encouragement from people who have used this
successfully in mouse cells especially primary transgenic immune cells.

I am purchasing the FDG from Molecular Probes. Any help would be useful.

Thanks in advance,
Rochelle Diamond
California Institute of Technology
Division of Biology
Flow Cytometry/Cell Sorting Facility
diamond@cco.caltech.edu

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu