Life expectancy of FACS nozzles

DAVIN JUTILA (SM0973%SPRINGB.SIUCVMB@SIUCVMB.SIU.EDU)
Wed, 19 Jul 95 11:47:21 CST

*-------------------- FLOW CYTOMETRY FACILITY

Hello again,

Thank you to all who responded to my inquiry into protein secretion.

This is a question I have been wanting to ask, but always forget when I
send a posting.

Is there a life expectancy for the nozzles on the FACS Vantage (FACstar Plus)?
We have had our Vantage for about two years and have three 70um tip that I'm
unable to get tight CV's and short stream break off points for sorting. I'm
I not cleaning the nozzle good enough? or do the nozzles wear down where their
orfice is rough and produces turbulence.These nozzles are very expensive and
any response would be appreciated.

Thank you in advance.

Davin Jutila

DAVIN JUTILA, DIRECTOR SIU SCHOOL OF MEDICINE
FLOW CYTOMETRY FACILITY-1220 SPRINGFIELD, IL 62702
PHONE: (217)-524-5812 FAX: (217)-524-3227
EMAIL: SM0973%SPRINGB@SIUCVMB.SIU.EDU

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