Re > Protein secretion
Monika (Monika@miltenyibiotec.de)
19 Jul 1995 17:15:47 +0200
Nachricht von Re > Protein secretion
R. Manz, M. Assenmacher, E. Pflueger, S. Miltenyi and A. Radbruch from the
University of Cologne published a paper describing a method which should help
you. It's titled "Analysis and sorting of live cells according to secreted
molecules, relocated to a cell-surface affinity matrix" and was published in
Proc. Natl. Acad. Sci. USA, Vol 92, pp. 1921-1925.
They create an artificial matrix on the cells, specific for the secreted
molecule. Cells are embedded in gelatine to prevent crossfeeding of secreted
products to other cells. Subsequently they sorted the cells magnetically by
using antibodies against the secreted product conjugated to magnetic microbeads.
Good luck,
Alexander Horst
e-mail AlexanderH@MiltneyiBiotec.de
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
