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Hi Mikael,
as far as I know, there is no way to get the instrument settings from the
FCS Datafile, they are not stored in the datafile anymore...
Here is a typical header from a Cellquest data file:
FCS2.0 256 1591 1792 51791 0 0
\$BYTEORD\4,3,2,1\$DATATYPE\I\$NEXTDATA\0\$SYS\Macintosh System Software
7.1.0\CREATOR\CELLQuest
1.1.1\$TOT\5000\$MODE\L\$PAR\5\$P1N\FSC-H\$P1R\1024\$P1B\16\$P1E\0,0\$P2N\SS
C-H\$P2R\1024\$P2B\16\$P2E\4,0\$P3N\FL1-H\$P3R\1024\$P3B\16\$P3E\4,0\$P4N\FL
2-H\$P4R\1024\$P4B\16\$P4E\4,0\$P5N\FL3-H\$P5R\1024\$P5B\16\$P5E\4,0\$CYT\FA
CScan\$BTIM\16:52:33\$ETIM\16:52:43\BD$AcqLibVersion\1.1.1\BD$NPAR\5\BD$P1N\
FSC-H\BD$P2N\SSC-H\BD$P3N\FL1-H\BD$P4N\FL2-H\BD$P5N\FL3-H\
and there are not enough instrument settings defined !!!!
I don't understand either why BDIS is not following the FCS2 Standard,
especially as on the Mac, Data file size is not really a problem (with
optical disks being much cheaper than anything on the HP). I hope they are
going to reverse this soon !! This doesn't mean that they can't keep a
separate settings file as they do now in Cellquest, but at least one could
get the settings back from the datafiles as well..
There is several other stupid things in Cellquest (in case the Programmers
in BDIS USA are listening to this mailing list ??????? Maybe they'll change
this in the next update !!!):
1. Why didn't they integrate the FACSConvert in the program, so that one
can read FCS 1.0 and FCS 2.0 files directly ??? Only to ask people to pay a
couple of hundred $ extra ???
2. Why didn't they follow some normal intuition ??? eg. using duplication
(copy/paste) to create plot with the same size, allow change of format for
each plot (Histo into Dot etc) and so on.. I have a long list of requests..
3. Why can't one modify the Axis labels after exporting to Claris Works...
4. Why do they sell Quickkeys if it is incompatible with Cellquest (exept
of the most stupid macros, everything a bit more complicated crashes
Cellquest...).
5.-100. I don't want to go into more details, just suggesting that we as
users should try to convince the programmers to take into account our
critics !!! Especially as Cellquest is listed for more than 5000 $ here in
France !!!
I would hope that if we are many to complain about the same things, maybe
we can create a wishlist for BDIS (at least until someone comes up with a
free program on the Mac that is more suited for scientific use...).
Matthias
______________________________________________________________
Dr. Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry Laboratory _/ _/ _/ _/ _/
Department of Immunology _/ _/_/_/ _/_/_/
Institut Pasteur _/ _/ _/
25 rue du Dr. Roux _/_/_/ _/ _/
F - 75724 PARIS Cedex 15 INSTITUT PASTEUR PARIS
France
Tel: 33 1 40 61 31 29
Tel: 33 1 45 68 85 38
Fax: 33 1 45 68 86 39
Email: mhaury@pasteur.fr
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